Abstract: Carbon nitride support (CN) was synthesized by high-temperature calcination using melamine, cyanuric acid, and citric acid as raw materials, and then Ru/CN supported single atom catalyst was prepared by impregnation reduction method. Utilize various characterization methods to investigate the structural composition and microstructure of catalysts, and then investigate the influence of different factors on the rate of hydrogen production by hydrolysis of ammonia borane (NH3BH3, AB). The results showed that the lattice stripe spacing of the catalyst was 0.211 nm, corresponding to the (002) crystal plane of Ru, indicating that Ru nanoparticles were successfully loaded on the surface of the CN carrier. The TOF value of Ru/CN catalyst with a Ru loading of 0.05 mmol is 446.4 min-1. The Ru/CN catalyzed AB hydrolysis hydrogen production reaction can be approximately regarded as a first-order reaction relative to the amount of catalyst used; heating up can increase the effective collision frequency between reactant molecules, promoting easier hydrogen production reactions; and through calculation and analysis, it is shown that the concentration of AB has a positive effect on the Ru/CN catalyzed AB hydrolysis hydrogen production reaction, with an activation energy of 53.6 kJ/mol. After 5 cycles of use, the Ru/CN catalyst can still catalyze AB complete hydrolysis hydrogen production.