In this paper, the effect of temperature on the creep properties and microstructure of nickel-based superalloy GH4169 were studied, and its fracture morphology and creep rupture mechanism were analyzed. The experimental results showed that the steady creep rate of GH4169 alloy increased and the creep life of GH4169 alloy decreased significantly with the increased of creep temperature, that was, the alloy had strong temperature sensitivity. During the creep process, the γ" phase grew, aggregated and transformed to the δ phase. With the increased of creep temperature , the transition of γ″ phase to the δ phase was faster, the amount of γ″ phases in the crystal decreased, and the size and area of the δ phase increased, and the number and size of secondary cracks decreased. When the creep temperature was 650 ℃, there were more bright white tearing edges in the fracture, the dimple size was different, there were a small amount of precipitates and carbonization When the temperature increased to 670 ℃, the dimple size decreased, mainly shallow dimples, and cleavage surfaces appeared; when the temperature increased to 690 ℃, there were only a few dimples and cleavage steps, and the number of δ phases increased significantly, which meant that fracture mode was cleavage fracture or quasi-cleavage fracture.