时国庆, 郭彦峰, 魏巍, 宋青, 张怀, 钟广蓉. 雌激素受体ERβ配体结合区的异源表达与纯化[J]. 工程科学学报, 2007, 29(S2): 208-211. DOI: 10.13374/j.issn1001-053x.2007.s2.087
引用本文: 时国庆, 郭彦峰, 魏巍, 宋青, 张怀, 钟广蓉. 雌激素受体ERβ配体结合区的异源表达与纯化[J]. 工程科学学报, 2007, 29(S2): 208-211. DOI: 10.13374/j.issn1001-053x.2007.s2.087
shu
SHI Guoqing, GUO Yanfeng, WEI Wei, SONG Qing, ZHANG Huai, ZHONG Guangrong. Heterologous expression and purification of the ligand binding domain of estrogen receptor β[J]. Chinese Journal of Engineering, 2007, 29(S2): 208-211. DOI: 10.13374/j.issn1001-053x.2007.s2.087
Citation: SHI Guoqing, GUO Yanfeng, WEI Wei, SONG Qing, ZHANG Huai, ZHONG Guangrong. Heterologous expression and purification of the ligand binding domain of estrogen receptor β[J]. Chinese Journal of Engineering, 2007, 29(S2): 208-211. DOI: 10.13374/j.issn1001-053x.2007.s2.087
shu

雌激素受体ERβ配体结合区的异源表达与纯化

Heterologous expression and purification of the ligand binding domain of estrogen receptor β

    摘要
  • 摘要: 为研究环境化合物与雌激素受体的相互作用,在大肠杆菌中异源表达了人雌激素受体ERβ的配体结合区(hERβ-LBD)蛋白。在诱导剂(IPTG)浓度为0.2mmol·L-1,培养温度25℃,诱导时间为8h的优化表达条件下,经亲和色谱法纯化后,hERβ(LBD蛋白的产量可达236mg·L-1.

     

    Abstract: In order to investigate the interaction between environmental chemicals and estrogen receptors,the ligand binding domain of human estrogen receptor β(hERβ-LBD) was expressed in Escherichia coli.Based on the optimized expression conditions(IPTG,0.2 mmol·L-1;culture temperature,25℃;time of induction,8 h),after purified by affinity chromatography,the production of hERβ-LBD can be reached to 236 mg·L-1.

     

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