Heterologous expression and purification of the ligand binding domain of estrogen receptor β

SHI Guoqing; GUO Yanfeng; WEI Wei; SONG Qing; ZHANG Huai; ZHONG Guangrong

doi:10.13374/j.issn1001-053x.2007.s2.087

SHI Guoqing, GUO Yanfeng, WEI Wei, SONG Qing, ZHANG Huai, ZHONG Guangrong. Heterologous expression and purification of the ligand binding domain of estrogen receptor β[J]. Chinese Journal of Engineering, 2007, 29(S2): 208-211. DOI: 10.13374/j.issn1001-053x.2007.s2.087

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Heterologous expression and purification of the ligand binding domain of estrogen receptor β

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In order to investigate the interaction between environmental chemicals and estrogen receptors,the ligand binding domain of human estrogen receptor β(hERβ-LBD) was expressed in Escherichia coli.Based on the optimized expression conditions(IPTG,0.2 mmol·L^-1;culture temperature,25℃;time of induction,8 h),after purified by affinity chromatography,the production of hERβ-LBD can be reached to 236 mg·L^-1.

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Heterologous expression and purification of the ligand binding domain of estrogen receptor β

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